Cloning, expression, and characterization of a self-sufficient cytochrome P450 monooxygenase from Rhodococcus ruber DSM 44319

Cloning, expression, characterization and engineering of cytochrome P450 CYP116B3 from Rhodococcus ruber DSM

Cloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium

Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...

Cloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium

Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...

Expression, purification and kinetic characterization of recombinant benzoate dioxygenase from Rhodococcus ruber UKMP-5M

In this study, benzoate dioxygenase from Rhodococcus ruber UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (benA gene) from Rhodococcus ruber UKMP-5M was then expressed, purified, characterized, The benA gene was amplified (642 bp), and the product was cloned into a pGEM-T vector.The recombinant plasmid pGEMT-benA was digested by d...

cloning and gene expression of cytochrome p450 gene from alcanivorax borkumensis bacterium

alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. the ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. a. borkumensis have two genetic systems for alkane biodegradation. the first system is alkane hydroxylase (alk-b1and alk-b2) and the second system is  cytochrome p450....